Cardiac reporter gene imaging using the human sodium/iodide symporter gene
نویسندگان
چکیده
منابع مشابه
Reporter gene imaging using radiographic contrast from nonradioactive iodide sequestered by the sodium-iodide symporter.
The hypothesis that the human sodium-iodide symporter, NIS, can be used to detect NIS expression using standard radiological techniques was tested using adenoviral transduced NIS expression in human tumor xenografts grown in mice and in a naive dog prostate. Nonradioactive iodide was administered systemically to animals that 1-3 days previously had received a local injection of a replication-co...
متن کاملThe Sodium Iodide Symporter (NIS) as an Imaging Reporter for Gene, Viral, and Cell-based Therapies
Preclinical and clinical tomographic imaging systems increasingly are being utilized for non-invasive imaging of reporter gene products to reveal the distribution of molecular therapeutics within living subjects. Reporter gene and probe combinations can be employed to monitor vectors for gene, viral, and cell-based therapies. There are several reporter systems available; however, those employin...
متن کاملA "new" reporter in the field of imaging reporter genes: correlating gene expression and function of the sodium/iodide symporter.
By 1995, more than 150 human gene therapy clinical trials were in progress in the United States alone (1). More than 10 y later, gene delivery in humans remains a considerable challenge for numerous potential applications, because it is often limited by poor delivery specificity, inadequate transfer efficiency, and lack of correlation between expression and function. The limitations of clinical...
متن کاملOptical imaging of cardiac reporter gene expression in living rats.
BACKGROUND Studies of cardiac gene transfer rely on postmortem analysis using histologic staining or enzyme assays. Noninvasive imaging of the temporal and spatial characteristics of cardiac gene expression in the same subject offers significant advantages. METHODS AND RESULTS Rats underwent direct myocardial injection via left thoracotomy with adenovirus-expressing firefly luciferase (Ad-CMV...
متن کاملCorrelation of Na+/I- symporter expression and activity: implications of Na+/I- symporter as an imaging reporter gene.
UNLABELLED The Na(+)/I(-) symporter (NIS) has been proposed as an imaging reporter gene to ascertain the expression of therapeutic genes in targeted tissues. In this study, we investigated whether posttranslational processing and cell-surface trafficking of NIS affect NIS-mediated radioiodide uptake in cells expressing exogenous NIS. METHODS We established FTC133, HeLa, and PC12 cell lines wi...
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ژورنال
عنوان ژورنال: Cardiovascular Research
سال: 2005
ISSN: 0008-6363
DOI: 10.1016/j.cardiores.2004.10.001